Sponsor Lunch Sessions
Thursday, May 9
| Sponsor | Time | Location | Talk Title | About |
| Beckman Coulter | 12:05-12:50 | Courtyard | Expanding horizons in isolation and characterization of extracellular vesicles (EVs) | Join us as we delve into the intricacies of EV characterization, presenting a workflow designed to shed light on EVs. Throughout this presentation by our team of experts, we will explore the advantages and limitations of various centrifugation techniques such as differential centrifugation, density gradient ultracentrifugation, and more. The downstream characterization of the EV fraction is often performed using bulk analysis. We will discuss the significance of deciphering the heterogeneity of EVs and explore the methods that can be employed for this purpose. This includes analytical ultracentrifugation (AUC), which determines the sample purity, detects contaminants and aggregates, size distributions, anisotropy, and sedimentation and diffusion coefficients. In the realm of single-EV analysis, flow cytometry emerges as a game-changer. We will address the technological challenges associated with conventional flow cytometry and the importance of standards and controls. Finally, we will introduce the CytoFLEX nano design, which redefines nanoparticle analysis and facilitates usability, even for those new to flow cytometry. It expands the horizon of EV characterization with increased sensitivity on scatter and fluorescence. |
| Particle Metrix | 12:05-12:50 | 111-112, Level 1 | Characterization of Extracellular Vesicles and other biological nanoparticles using Nanoparticle Tracking Analysis (NTA) | Nanoparticle Tracking Analysis (NTA) has emerged as a fast and vital characterization technology for Extracellular Vesicles (EVs), Exosomes and other biological material in the size range from 30 nm to 1 μm. While classic NTA scatter operation feeds back the size and total particle concentration, the user typically cannot discriminate whether the particle is a vesicle, protein aggregate, cellular trash or an inorganic precipitate. The fluorescence detection capabilities of f-NTA however enables the user to gain specific biochemical information for phenotyping of all kinds of vesicles and viruses. Alignment-free switching between excitation wavelengths and measurement modes (scatter and fluorescence) allow quantification of biomarker ratios such as the tetraspanins (CD63, CD81 and CD9) within minutes. Furthermore, specific colocalization studies using c-NTA gives a deeper understanding of the composition of biomarker on single particle. |
| NanoFCM | 12:05-12:50 | 104, Level 1 | The Next Generation of Flow NanoAnalyzer | Join us for a lunch and learn session as we present the next generation of Flow NanoAnalyzer, the Flare and Auto20.We will cover the features and capabilities in depth, sharing example data from a variety of EV applications. Included in the presentation will be applications of our new software quantification features and automated sample loading and cleaning on the Auto 20. Please join us to learn about the future of nano flow cytometry for EVs. |
Friday, May 10
| Sponsor | Time | Location | Talk Title | About |
| Evident Australia and Abbelight | 12:05-12:50 | Courtyard | Unveiling the unseen: Visualize your EVs with Abbelight and Evident | Leveraging over a century of leadership in optical microscopy system development, Olympus Scientific Solutions, now operating under the name Evident, in partnership with the pioneering nanoscopy innovator Abbelight, is excited to extend an invitation to explore the nano-world of Extracellular Vesicle imaging.Abbelight's comprehensive solution offers a seamless pipeline for EV characterization, encompassing every step from sample preparation to intricate data analysis. With the AbbelightTM Smart EVs Kit, researchers are empowered to effortlessly and precisely prepare and stain their samples. The cutting-edge nanoscopy SAFe platform facilitates high-quality, high-throughput imaging. Furthermore, the advanced data analysis software enables scientists to accurately assess the size, protein composition, and morphology of individual EVs with unparalleled ease.In addition, we invite you to participate in our raffle for an opportunity to win a comprehensive characterization of your EV sample using Abbelight and Evident technologies. |
| IZON | 12:05-12:50 | 111-112, Level 1 | You’re invited: Reset and refresh with Izon Science | Hi! We are super excited to attend this year’s ISEV event and have been gifted some precious time and space to work with during your Friday lunchtime. We appreciate conferences are pretty energy intensive, and there may be other exciting activities happening at the same time! So, we are planning to keep this light and lowkey, with prizes to be won – and we won’t ask for all of your time here. Come along to our 5-minute presentation on the Friday morning, and we will share details with you on the day. And of course, be sure to visit our booth to say hi and see the latest developments in qEV isolation with your own eyes! |
| ONI | 12:05-12:50 | 104, Level 1 | Simplifying Super-Resolution: Automating Your Sample to Answer Journey with ONI | Join us for an enlightening talk as ONI, a pioneering tech company, takes center stage to discuss the transformative power of a streamlined workflow in extracellular vesicles (EV) research. At the heart of this, is ONI’s newest products EV Profiler 2 Application Kit™, the software AutoEV, and our upcoming product, Aplo Flow, which offer a revolutionary solution that redefines EVs research using super-resolution microscopy. The workflow ensures accessibility for scientists of all levels, simplifying the super-resolution process. Aplo Flow seamlessly integrates application-specific sample preparation with fully automated fluidic control. EV Profiler 2 allows scientists to analyze EV phenotype, size and cargo at a single EV level, while AutoEV's streamlined workflow lets even novice users navigate effortlessly from experiment setup to automated image acquisition and analysis, reducing the complexities of EV data collection and reporting. Start your journey with Aplo Flow, EV Profiler 2 and AutoEV, ONI’s state-of-the- art ‘sample to answer’ platform to automate EV super-resolution research. |
Saturday, May 11
| Sponsor | Time | Location | Talk Title | About |
| Fujifilm | 12:05-12:50 | Courtyard | In-depth proteome profiling of EVs for quality assessment and liquid biopsy development, Koji Ueda Cancer Precision Medicine Center, Japanese Foundation for Cancer Research | Numerous studies on EVs in recent years have led to great interest not only in elucidating their basic biological functions, such as biosynthetic pathways and functions, but also as diagnostic and therapeutic tools. In fact, liquid biopsy targeting EV-encapsulated molecules and therapies using MSC-derived EVs are approaching practical application.
These tools are expected to further accelerate basic research and clinical applications of EVs. |
| HansaBioMed & Microgentas | 12:05-12:50 | 111-112, Level 1 | An Innovative Fusion of Two Filtration Technologies: Combination of size exclusion techniques (TFF) and charge-based filtration for improved quality of EVs from complex biofluids. | The synergistic combination of Tangential Flow Filtration (TFF) and ExoFilter, a charge-based filtration technology, has demonstrated great advantages in isolating EVs from complex biofluids, like plasma or serum. This fusion of two filtration methods has transcended the limitations of current EVs extraction techniques, achieving good particle yield with an user friendly workflow and reducing the levels of contaminants often co-isolated with EVs. .Join our sponsored lunch session and live practical demonstration to witness firsthand how TFF-EVs from HansaBioMed and ExoFilter from Microgentas have accomplished this innovative breakthrough. |
| Horiba | 12:05-12:50 | 104, Level 1 | 5 Things to Consider Before Selecting an NTA | NTA is a well-known method in the EV community that has been around for decades. It is a dark field microscopy that tracks particle movements to infer size and concentration. However, every commercialized NTA is designed differently. How do you know what to look for in the current landscape? What procedures should you follow to handle poorly understood samples like EV’s. How do you confirm repeatability and reproducibility for your measurements? That is how to suppress cross contamination and what buffers work best? How do you cost effectively manage a multi-user system and address issues like readiness for the next user? Join us to listen, ask questions, and add your own comments. |
| Secretech, Inc | 12:05-12:50 | 105-106, Level 1 | High-throughput single Extracellular Vesicle (EV) Profiling enabled by Secretech’s Proximity Barcoding Assay (PBA) | Join us to discover how Secretech’s novel technology is revolutionising profiling of extracellular vesicles (EVs) from bulk analysis down to single-particle resolution. Detection and characterisation of their proteins at single EV level provides insights into the heterogenic nature of EVs, especially in body fluids with EVs secreted from diverse cell of origins. Our Proximity Barcoding Assay (PBA) reveals the expression of hundreds to thousands of proteins at single EV level. An atlas of EV subpopulations can then be constructed to visualise and analyse EV heterogeneity, thus enabling biomarker discovery at new resolutions. You are welcome to Secretech’s lunch session to find out how our technology can accelerate your research! |